Tissue Specific Esterase Isozyme Variation in Ocellated Pufferfish, Tetraodon cutcutia
Sabekun Nahar 1, Md. Abdur Rashid
Variation in the expression of esterase isozymes was studied from twenty different tissues of Tetraodon cutcutia on 7.5% polyacrylamide gel electrophoresis (PAGE) stained with a and b naphthyl acetates. A variant type of allelic expressions was observed in different tissues where altogether, five esterase bands (Est-1, Est-2, Est-3, Est-4 and Est-5) were detected. Est-4 showed the highest frequency (95%) whereas Est-3 was found to be lowest (30%). Localization of esterase isozymes were also observed in different tissues that showed tissue specific isozyme variation. Tissues that were related to digestive system (liver, intestine) showed higher allelic variation (80%). All the bands were present only in eye tissue. One the other hand, only one band (Est-4) was present in anterior_ and mid_muscle. Est-3 was absent in digestive, nerve and muscle tissues but was present in skin, fin, eye and adipose tissues.