Entomopathogenic nematodes have been developed as the new hope for insect pest management. Four isolates of entomopathogenic nematodes tagged as CS1, CS21, CH5 and CH6 were recovered from the soil of Saharanpur District of Uttar Pradesh, India, two of each genus Steinernema and Heterorhabditis respectively and were tested for their pathogenicity and recyclingrnpotential against Galleria mellonella larvae. Results revealed that isolate CH6 was highly virulent and caused 100% mortality within 48 h followed by CS21 and CH5 within 60 hours. A positive correlation was found between LC50 and LT50 values where increment in LT50 lead to the increment in LC50 values. The recovery of IJs/larva was 3.1±0.13×105, 2.16±0.1×105, 1.68±0.08×105and 1.44±0.1×105 with CS1, CH5, CH6 and CS21 respectively. Results revealed that all the isolates are highly virulent with an excellent reproductive potential in laboratory condition and can be applied further for pest management and commercialization.
Istkhar, Ashok Kumar Chaubey, Aasha, Aashaq Hussain Bhat. Virulence and recycling potential of entomopathogenic nematodes (Nematoda: Steinernematidae, Heterorhabditidae) from Saharanpur district, western Uttar Pradesh, India. Journal of Entomology and Zoology Studies. 2016; 4(1): 27-32.