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Journal of Entomology and Zoology Studies
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ISSN Print: 2349-6800 | ISSN Online: 2320-7078

Journal of Entomology and Zoology Studies

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2016, Vol. 4, Issue 6
Immunomodulatory effect of chlorpyrifos formulation (Pyrifos- 20 EC) on Philosamia ricini (Lepidoptera: Saturniidae)

Moni Kankana Kalita and Dipali Devi

In present study immunotoxic effect of organophosphate pesticide, chlorpyrifos formulation (Pyrifos-20 EC) on Philosamia ricini (eri silkworm) was evaluated. Herein, two sub lethal concentrations of chlorpyrifos (1.5 mg/L and 2.0 mg/L) were administered to eri silkworm and the effect on Phenoloxidase (PO), lysozyme enzyme activity and hemocyte immunity were investigated at 24-96 h time interval. Results showed at 72-96 h 2.0 mg/L concentration showed significant inhibition in enzyme activity. Likewise, after 24 h of exposure a significant increase in lysozyme activity was recorded in both pesticide exposed groups, however at 72-96 h significant decrease in lysozyme activity was measured. Moreover, after 48-96 h significant reduction in hemocyte abundance was observed (p<0.05). Changes in proportional counts of hemocytes showed that sub lethal chlorpyrifos exposure caused an increase of granulocytes and plasmatocytes in a concentration-dependant manner. However, prohematocytes, spehrulocytes and oenocytes abundance was decreased significantly (p<0.05) in chlorpyrifos exposed groups.
Pages : 26-31 | 621 Views | 28 Downloads
How to cite this article:
Moni Kankana Kalita and Dipali Devi. Immunomodulatory effect of chlorpyrifos formulation (Pyrifos- 20 EC) on Philosamia ricini (Lepidoptera: Saturniidae). 2016; 4(6): 26-31.
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