author(s)
Muhammad Junaid, Abu Nasar Siddique, Muhammad Tariq Masood Khan, Ibrar Alam, Muhammad Waqas, Saba, Safia Hameed and Wali Muhammad

abstract
This multidimensional study was conducted on three hundred and ninety-six (396) hemophilia patients (331 males and 65 females). The hemophilia patients were screened for HBsAg, anti-HCV antibodies, and anti-HIV antibodies by using automated advanced examination diagnostical technique that is Chemiluminescent Microparticle Immunoassay (CMIA) to detect HBV, HCV, and HIV infections. The blood samples were collected by venipuncture and the serum was separated by centrifugation. After that, all the samples were run on ARCHITECT i1000SR automated immunoassay analyzer Chemiluminescent Microparticle base technology to get results. Out of 396 blood samples, we have investigated in this study, 72 (18%) were positive for HCV infection and 12 (3%) were confirmed to be positive for HBV infection. HIV infection was not detected in any patient. Our study demonstrates that the prevalence of HCV amongst Pakistani donors is highest while HBV is on a decline.