author(s)
Milind B Katare, WS Lakra, NK Chadha, Manjulesh Pai, N Basavaraja, Subodh Gupta, Paramita Banerjee Sawant, Sambid Swain and Mukesh Kumar

abstract
In the present investigation, 17α-methyltestosterone (MT) treatment at 50, 100, 150 and 200 ppm (mg/kg feed) to 3-day-old T. lalius, significantly produced highest masculinization than the control. At the highest dose (200 ppm) of MT produced maximum male percentage (85.32%). Indirect feminization also observed through crossing between sex reversal males with ordinary female. The GSI and histological examination showed the appearance of suppressed atretic oocytes. The hormonal profiling clearly revealed that, the MT treatment induced stress and produce the cortisol, which actively involved in the testosterone production and estradiol reduction.