Molecular characterization of luteinizing hormone receptor (LHR) gene in Murrah bulls
JK Reen, KP Ramesha, Preeti, SD Revanasiddu and K Rohit
Semen quality of bulls is commonly affected due to multitude inherent genetic disorders. Gonadotropins, interacting with their gonadal receptors, play a key role in sexual development, reproductive functions and metabolism. In this study, we performed molecular characterization and detection of genetic polymorphism in Exon 1, Exon 2, Exon 3, Exon 4 and Exon 5 regions of Luteinizing Hormone Receptor (LHR)gene in Murrah bulls. Genomic DNA was isolated from the blood samples within 24 hours of collection by high salt method. Polymorphism was studied using Polymerase Chain Reaction-Single Stranded Conformational Polymorphism (PCR-SSCP) technique followed by Sanger Sequencing. PCR-SSCP and sequence analysis revealed monomorphism within the studied population that is coding region as well as exon-intron boundaries of Exon 1, Exon 2, Exon 3, Exon 4 and Exon 5 of Luteinizing Hormone Receptor (LHR)gene is highly conserved among Murrah bulls. Consequently, we could not perform association studies with fresh and frozen semen quality parameters in Murrah bulls.
JK Reen, KP Ramesha, Preeti, SD Revanasiddu and K Rohit. Molecular characterization of luteinizing hormone receptor (LHR) gene in Murrah bulls. Journal of Entomology and Zoology Studies. 2018; 6(3): 246-250.