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Journal of Entomology and Zoology Studies
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ISSN Print: 2349-6800 | ISSN Online: 2320-7078

Journal of Entomology and Zoology Studies

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2018, Vol. 6, Issue 4
Nucleotide base variations in aligned sequences of ΑS1, ΑS2, Β and K-casein gene along with regression study of different milk composition traits in Malvi, Nimari, Sahiwal and HF crossbred cows

Akhilesh Pandey and MS Thakur

The aim of the present study was to evaluate the nucleotide variation by sequencing of PCR product of αs1, αs2, β and κ-casein gene of Malvi, Nimari, Sahiwal and HF crossbred cattle. In alignment of nucleotide sequences of αs1, αs2 and κ-casein gene in Malvi, Nimari, Sahiwal and HF crossbred cows revealed the nucleotide variation. The align sequence of αs1 casein gene of HF crossbred, Nimari and Malvi cattle showed similar nucleotide base sequence, whereas in Sahiwal the Guanine (G) at 44 bp site was replaced by Adenine (A). For αs2 casein gene, the HF crossbred, Sahiwal and Malvi showed similar nucleotide base sequence whereas in Nimari the Adenine (A) at 243 bp site was substituted by Thymine (T) and at 582 bp site in HF crossbred Adenine was replaced by Guanine. In same αs2 gene Thymine (T) at 713 and 715 bp site was replaced by Cytosine. There was no change observed in 121 bp of β casein gene in HF crossbred, Sahiwal, Nimari and Malvi. For K- casein gene, the Nimari, Sahiwal and Malvi showed similar nucleotide base sequence, whereas in HF crossbred, the Cytosine (C) at 262 bp site was substituted by the Thymine (T) and at 308 bp site Adenine (A) was replaced by Guanine in Nimari. The phylogenic tree represented that the four breeds were divided in to four clusters. The clusters at αS1, αS2 and K gene locus in Sahiwal and Nimari showed more genetic divergence with HF crossbred as compared to the Malvi, whereas at β gene locus only Nimari showed more genetic divergence with HF crossbred as compared to Malvi and Sahiwal.
Pages : 15-19 | 192 Views | 38 Downloads
How to cite this article:
Akhilesh Pandey and MS Thakur. Nucleotide base variations in aligned sequences of ΑS1, ΑS2, Β and K-casein gene along with regression study of different milk composition traits in Malvi, Nimari, Sahiwal and HF crossbred cows. 2018; 6(4): 15-19.
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