In vitro and in-vivo efficacy of Eucalyptus citriodora Leaf in gastrointestinal nematodes of goats
Sushmita Sastya, Rajeev Ranjan Kumar and Stuti Vatsya
The study was undertaken to evaluate the anthelmintic activity of crude powder (CP), crude aqueous (CAE) and crude methanolic (CME) extracts of Eucalypyus citriodora leaves. In vitro anthelmintic activity was evaluated against freshly collected adult Haemonchus contortus using Adult Motility Test (AMT). However, against eggs and larvae of gastrointestinal nematodes of goats using Egg Hatch Assay(EHA) and Larval Paralysis Test(LPT) at 0.156%, 0.312%, 0.625%, 1.25%, 2.5%, 5% and 10% concentration. In-vivo trial was conducted in goats naturally infected with GI nematodosis by using Faecal Egg Count Reduction Test (FECRT%). In AMT an average highest corrected motility of 100% was observed from 1.25–10% followed by 66.66%, 46.66%, 46.66% at 0.625%, 0.312 and 0.156% concentration, respectively. In EHA, CAE showed better ED50=0.997mg/mland ED99=105.033 mg/ml values than CME (ED50= 1.820 and ED99= 52.182 mg/ml). Both CAE and CME showed highest (100%) inhibition of egg hatching at 50 and 100 mg/ml and minimum (60% and 54%) at 1.56 mg/ml concentration, respectively. In LPT, CAE (ED50= 5.305 and ED99= 157.326mg/ml) and CME (ED50= 5.503 and E99= 165.447 mg/ml) showed highest 100% and 100% and lowest 25% and 22% paralysis of third stage larvae of gastrointestinal nematodes at 100mg/ml and 1.56 mg/ml concentration, respectively. In in-vivo anthelmintic activity, CMEshowed highest (35%) reduction in faecal egg count followed by CAE (21.05%) and then CP (10.34%) @ 4gm/kg b.wt. on 7, 14 and 14 day post-treatment, respectively.