A review for transgenic mouse model creation and its use in drug discovery
Dr. Satheesh Kumar P and Dr. Karthiga S
Introduction of human disease trait genes into the mouse germ line is considered a major technical advancement in the fields of developmental biology and genetics. This technology now referred to as transgenic mouse technology has revolutionized virtually all fields of biology and provided new genetic approaches to model many human diseases. There were many transgenic lines with expression of foreign genes specifically targeted to desired organelles/cells/tissues have been characterized. In GLP standard in vivo laboratory facility housed knock out/in and transgenic mice. Right now, international institutions like Charles, Jackson labs are creating the new knock out/in and transgenic mice and supply those to worldwide. Moreover, cost of each model is very high and we paying in terms of dollars. More importantly, we have lagging of facilities to maintain and preserve the germ lines of existing transgenic mice. In this review we explain how to create transgenic and compared with different techniques of gene editing.