An experiment was conducted to evaluate the thermal tolerance limit and oxygen consumption rates of Labeo gonius (6.23±0.19 g) fingerlings acclimated to four different temperatures (27, 30, 33 and 36 °C) for a period of 60 days. Critical thermal maxima, CTmax (40.97±0.05, 41.89±0.05, 42.69±0.04and 43.05±0.05) and critical thermal minima, CTmin (12.72±0.04, 13.62±0.04, 13.99±0.05 and 14.39±0.021) increased significantly (p<0.05) with increasing acclimation temperatures. Similarly, Lethal thermal maxima, LTmax (41.63±0.04, 42.41±0.05, 42.99±0.02 and 43.42±0.04) and Lethal thermal minima, LTmin (12.24±0.03, 13.25±0.03, 13.68±0.05 and 13.97±0.04) increased significantly (p<0.05) with increasing acclimation temperatures. A thermal tolerance polygon over the range of 27 to 36 °C had a calculated area of 256.24 °C2. Oxygen consumption rate increased significantly (p<0.05) with increasing acclimation temperatures. Similarly, oxygen consumption rates also significantly (p<0.05) differed without acclimating at different temperatures. Temperature quotient (Q10) was found to be 1.72 for acclimated fish and 2.23 for non-acclimated fish. From this study, it is evident that thermal tolerance limit of L. gonius is comparable to Indian major carps, indicating their potential for diversifying freshwater aquaculture.