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Journal of Entomology and Zoology Studies

 

Volume 1, Issue 6

 

Tissue tolerance to heat and cold shock in larvae of Sarcophaga ruficornis (Sarcophagidae: Diptera)

Author(s): Jaya Tripathi1, Uma Rani Agrawal2, Manisha Tripathi1, Raghav Ram Tewari3
1. Department of Zoology, Iswar Saran Degree College, University of Allahabad, Allahabad-211004, India.
2. Department of Zoology, CMP Degree College, University of Allahabad, Allahabad-211002, India.
3. Cytogenetics Research Laboratory, Department of Zoology, University of Allahabad, Allahabad-211002, India.

Abstract: Larvae of the flesh fly, Sarcophaga ruficornis were exposed to Heat and Cold shock stresses in the present study. One of the responses to stress is the internal tissue damage. Trypan blue dye exclusion method was used to analyze the tissue tolerance to heat shock and cold shock. There is a difference in tolerance level in nongut and gut tissues to these thermal stresses.

 

Figure of Trypan blue staining pattern in third instar larval tissues of S. ruficornis (a) Control (b) in vivo heat shock at 40 oC /60 min.

Trypan blue staining pattern in third instar larval tissues of S. ruficornis (a) Control (b) in vivo heat shock at 40 oC /60 min.


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