Journal of Entomology and Zoology Studies

Volume 2, Issue 2

Genetic variation of a migratory dragonfly characterized with random DNA markers

Author(s): Anulin Christudhas, Manu Thomas Mathai

Abstract: Polymorphism among individuals of dragonflies belonging to same genus and species was studied using molecular technique RAPD-PCR analysis. The RAPD banding pattern reflected the genetic diversity among Pantala flavescens. Reproducible and distinct polymorphic bands ranging approximately 200 bp to 2500 bp were generated with 5 RAPD primers. Operon c series primers OPC 7 and OPC 10 yielded unique bands of 1000 bp, 650 bp and 1100 bp which can be utilized for developing molecular markers for species identification specific for locations. The scoring pattern generated was utilized to construct the distance matrix using POPGENE 32 v1.31.

Fig: UPGMA dendrogram of genetic distance among 5 Indian populations of P. flavescens based on 5 RAPD primers.

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