Journal of Entomology and Zoology Studies
Volume 2, Issue 3
Effect of Manganese on haematological parameters of fish, Garra gotyla gotyla
Author(s): Jyoti Sharma and Seema Langer
Abstract: In the present study, an attempt has been made to study the effect of heavy metal Manganese (MnSO4) on the haematological parameters of fish Garra gotyla gotyla for 9 weeks experimental duration. 96hours LC50 value of Manganese for G. gotyla gotyla was found to be 3.2 mg/l and three concentrations viz. 20%, 40% and 60% of LC50 were employed during experimental period. The parameters evaluated include total erythrocyte count (TEC), haemoglobin (Hb), haematocrit (Hct), mean corpuscular volume (MCV), mean corpuscular haemoglobin (MCH), mean corpuscular haemoglobin concentration (MCHC), total leucocyte count (TLC) and differential leucocyte count (DLC). Compared to control groups, TEC, Hb and Hct were found to exhibit significant decline whereas MCV and MCH exhibited overall increase in all concentration groups while MCHC contrarily exhibited fluctuating pattern. TLC depicted significant increase in all fishes following exposure to Manganese. DLC was observed to register an increase in number of lymphocytes, monocytes and eosinophils but a decrease in neutrophils, basophils and thrombocyte population.
Fig: Microphotograph of blood smear of control G. gotyla gotyla showingerythrocytes (E), neutrophils (N), lymphocytes (L) and thrombocytes (Th) (100x).
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