Journal of Entomology and Zoology Studies

Volume 2, Issue 5

Humoral immune responses in DNA vaccine formulated with poly (methyl methacrylate) against Leishmania major

Author(s): Somayeh Zarrati, Fatemeh Maleki, Mehdi Mahdavi, Narges Khabaz zadeh Tehrani, Zahra Abrehdari Tafreshi, Amir hooman Asadi, Fatemeh Tabatabaie

Abstract: DNA vaccines can generate more powerful and broader immune responses than conventional vaccines. In order to increase immunity, the DNA vaccine has been supplemented with adjuvant. In this study a new nano-vaccine containing TSA recombinant plasmid and poly (methyl methacrylate) nanoparticles (acting as adjuvant) was designed and its immunogenicity tested on BALB/c mouse. After three intramuscular injections of nano-vaccine (100 µg), the recombinant TSA protein (20 µg) was injected subcutaneously. Finally as a challenge animals were infected by Leishmania major. After the last injection of nano-vaccine, after protein booster injection, and also after challenge antibody responses were evaluated by ELISA method. The findings of this study showed the new nano-vaccine was capable of inducing specific antibody responses.

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