author(s)
Rania Ali El Hadi Mohamed 1, Emadeldin Hassan Konozy 2, El Amin El Rayah

abstract
One year surveys were conducted at two sites in Khartoum State capital of Sudan: Hellat Kuku and Soba West. The study was focused into two species of mosquitoes: Aedes vexans and Culex quinquefasciatus. This selection was based on previous investigations indicated their being positive forRVF. The study aimed to investigate on RVFv dissemination inside two species mosquitoes in Khartoum State (Sudan) and investigation of the influence of climate on mosquitoes abundance. Weekly aspiration of wild mosquitoes has been conducted during 1st July 2011- 1st July 2012. The collected mosquitoes were identified by using classical keys. Data of climate were obtained from Sudan Meteorology Authorities. Males and females of the two mosquitoes species were divided into three cohorts: salivary glands, abdomen, and wings and legs. RVF Virus was therefore examined into twelve pools using Real- Time PCR technique. Results showed that Temperature, precipitation, and RH were significantly associated with the number of aspirated mosquitoes. The virus has been detected inside the pools of the abdomen and salivary glands of Ae. vexans mosquitoes. However, pools of salivary glands of Cx. quinquefasciatus were negative of the virus in spite of dissemination of the virus in the wings and legs. Based on these results it can be concluded that climatic factors affected on the number of aspirated mosquitoes during the study period. Ae. vexans mosquitoes exhibited an indicator of being competent to transmit the virus in contrast to Cx. quinquefasciatus.