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Journal of Entomology and Zoology Studies
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ISSN Print: 2349-6800 | ISSN Online: 2320-7078

Journal of Entomology and Zoology Studies

2014, Vol. 2, Issue 4
Deciphering the molecular phylogenetics of the Asian honey bee, Apis cerana and inferring the phylogeographical relationships using DNA barcoding

Rukhsana, K., Akhilesh, V. P. and Sebastian, C. D.

The Asian honey bee, Apis cerana are honey producers and pollinators of cultivated crops and wild plants. They occur in Asia, from Afghanistan to China and from Japan to southern Indonesia. A. cerana have yellow stripes on their abdomen and are habituated to Indian plains. These are less aggressive and also display less swarming behavior. Here we report the partial sequence of cytochrome oxidase sub unit I gene (COI) of A. cerana (GenBank Accession No. KM230116) and its phylogenetic relationship. The COI gene sequence of A. cerana showed considerable variation with other Apis species. The COI DNA barcode developed in this study can be used for accurate species identification. The COI partial coding sequence of A. cerana showed 2.72% difference over 513 bp nucleotides to A. cerana isolated from Indonesia (GU191875) and 6.04% to A. cerana isolated from Japan (AF153105). A. cerana demonstrates the efficiency of the barcoding gene in discriminating global phylogeographical variants among the Apis species complex.
Phylogenetic tree of Apis cerana using NJ method
Fig. 1: Phylogenetic tree of Apis cerana using NJ method
Pages : 218-220 | 1121 Views | 14 Downloads
How to cite this article:
Rukhsana, K., Akhilesh, V. P., Sebastian, C. D.. Deciphering the molecular phylogenetics of the Asian honey bee, Apis cerana and inferring the phylogeographical relationships using DNA barcoding. J Entomol Zool Stud 2014;2(4):218-220.
Journal of Entomology and Zoology Studies
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