The objective was to determine the population dynamics of Anopheles stephensi in relation to malaria transmission. The study was carried out in three villages of Bandar-Abbas's county, south of Iran, from April 2011 to March 2012. Mosquitoes were collected by Total catch, Human and Animal bait collection, Window trap, Pit shelters and CDC light traps. An. stephensi play as a dominant vector with endophagic and endophilic behavior. Ovary dissection revealed six dilatations indicate at least 9% of the population can reach to the dangerous age to potentially malaria transmission. Two peaks of blood feeding were observed, 9.00-10.00 P.M, and the 1.00-2.00 A.M. The gonotrophic cycle, survival rate, life expectancy of the species was 4, 0.82 and five days, respectively. Vectorial capacity was measured as 0.028. In conclusion, hot and wet climatic conditions support the persistence, density and longevity of An.rnstephensi could result in more significant indigenous malaria transmission.