16S rRNA gene sequencing of the most regnant and pernicious pests Brevundimonas diminuta, Craterium leucocephalum and Diachea leucopodia associated with dried preserved forensically valuable Calliphorids
Devinder Singh, Naina Khullar
Calliphorids are forensically most valuable insects. These are collected from the crime site as circumstantial evidence and preserved for the purpose of identification. Though the prima facie of these preserved specimens seems to be in the perfect well maintained state but molecular analysis on these specimens reveal that these are harboured by various pests that are internally calamitous to these specimens and degrade the intact DNA of these specimens into small fragments. Brevundimonas diminuta (bacterium), slime molds Diachea leucopodia, Craterium leucocephalum have been identified as the most regnant and pernicious pests and approximately 500bp of I6S rRNA gene of these have been sequenced for the correct identification of these pests from preserved insect specimens. The author has also attempted to espy the most inimical traits that these pests are equipped with which would in turn help in calibrating effective measures to avoid their infestation and in safeguarding these subtle specimens.