Vinblastine is an anti-microtubule drug used to treat certain kinds of cancer and Anbuta is a homeopathic medicine which are indicated on those diseases where the immune system is depressed. Aim of current study is assessment of mentioned medicines on breast cancerous cell line. The ZR75-1 cell line were treated with the different concentration of drugs and incubated for different times. The results demonstrate the highest toxicity of drugs at 1 mg/ml concentration for Anbuta and 100 µg/ml for Vinblastine after 72hrs incubation time. The results of current study was demonstrated the anti-cancer and protective activity of Anbuta as a potent homeopathic drug, against breast cancer.