The objective of this study was to determine some biochemical parameters, analyzed in two different Kosovo rivers. Fish samples were collected during March - July - October 2014 applying the electrofishing method alongside Sitnica and Lumbardhi i Prizrenit rivers. We have analyzed those biochemical parameters on 30 fish individuals on two fish species: Rutilus rutilus and Carassius gibelio. Analyses of these parameters werernconducted according these methods: determination and concentration of TP was analyzed with Biuret method, GLU was analyzed by Glucose Oxidase Enzymatic Method, while AST and ALT was analyzed with Enzymatic-Colorimetric Method, with kit (Roche) and analyzer used by model: Cobas Integra 400-Roche. Based on our results it was observed in Sitnica river were significantly changed (p<0.05) in level of total protein (TP), glucose levels (GLU), hepatic alanine transaminase (ALT) and aspartate transaminase (AST) compared with control group.