A new species of Chrysina is described from Guanajuato state, Mexico, including morphological and molecular characters based in genetic difference of cytochrome oxidase (cox1) mitochondrial marker. This new species belongs to a new called "Peruviana complex", which presents taxonomic problems due to the high morphological similarity and difficult allocation of the species C. peruviana. However the population of the Sierra de Santa Rosa de Lima, Guanajuato state, Mexico, has distinctive characters that separate it from all other populations.