Rearing and identification of Callosobruchus maculatus (Bruchidae: Coleoptera) in Chickpea
Fatima, Maqsood Shah, Amjad Usman, Kamran Sohail, Muhammad Afzaal, Bismillah Shah, Muhammad Adnan, Nazeer Ahmed, Khwaja Junaid, Syed Rizwan Ali Shah, Inayat-ur-Rahman
To study the rearing and identification of Callosobruchus maculates (Bruchidae: Coleoptera) in chickpea, the present research work was carried out at the Entomology Research Laboratory of The University of Agriculture, Peshawar, during 2014. C. maculatus, (Bruchidae; Coleoptera) is an important stored grain insect pest distributed in Asia and Africa, ranging from tropical to subtropical world. It has been reported that the favorite hosts for C. maculatus are cowpea and chickpea but it also feed on other legumes as well. Grains were collected from various godowns of village Maneri (Swabi) and were brought to the Research Laboratory of The University of Agriculture Peshawar. Grains were kept in jars at 28±2 ºC and 65±5% Relative humidity (RH) and covered with muslin cloth in order to supply adequate humidity to the grains. To establish the stock culture of C.maculatus chickpea was used as a host. Grains of Chickpea were kept in the jars and the jars were covered with muslin cloth and placed at 28.5 ºC and 67.25 RH. The parasitoid from the culture of C.maculatus was collected with the help of aspirator and was then preserved in 70% alcohol. The parasitoid was identified with the help of literature and specimens present in the Insect museum of the Department of Entomology, The University of Agriculture, Peshawar. Both the pest and the collected parasitoid were properly mounted and labeled for demonstration purposes and were then deposited in the Insect museum of the Department of Entomology, The University of Agriculture,rnPeshawar.