author(s)
Imran Khan, Hammad Hussain, Bismillah Shah, Waseem Ullah, Ahmad Naeem, Waqar Ali Khan, Nangial Khan, Muhammad Adnan, Khwaja Junaid, Syed Rizwan Ali Shah, Nazeer Ahmed, Mazhar Iqbal

abstract
Evaluation of differentrnculture media (Corn meal agar, water meal, potato dextrose agar, malt extractrnagar) for isolation and growth of Rhizoctonia solani and phytobiocidesrn(Neem oil, garlic, ginger, turmeric and pepper) to investigate their effectrnupon the inhibition of mycelial growth of R. solani was carried out inrnvitro. Maximum biomass (21.50 mg) was harvested from potato dextrose brothrnfollowed by malt extract broth (19.50 mg), while the lowest biomass (3.50 mg)rnwas produced by sterilized tap water. Food poison technique was used byrnamendment of phytobiocides in potato dextrose agar (PDA). Neem oil was used @ofrn0.1 ml/20ml potato dextrose agar medium, while the rest of phytobiocides werernadded @of 60mg/20ml of the medium. R. solani was inoculated asepticallyrnon the center of the phytobiocides amended medium. Results of the studyrnrevealed that all the phytobiocides reduced the colony diameters of the R.rnsolani significantly (P<0.05) except ginger, compared withrncontrol (Un-amended PDA medium). Garlic was found to be the most effectiverninhibitory phytobiocides restricting the colony diameter to (38.25mm) asrncompared with other tested plant products.