Effect of seed proteinaceous extracts on α-amylase activity of Carob moth, Ectomyelois ceratoniae Zeller (Lepidoptera: Pyralidae)
Mojtaba Esmaeily, Ali R Bandani, Saeed Farahani and Saeed Amini
The carob moth is a cosmopolitan pest of many agricultural commodities both in the field and in the storage. The aim of the current study was to investigate the effects of seed proteinaceous extracts against E. ceratoniae α-amylase. The results showed that bean, wild oat, pea, mung bean, barley and amaranth inhibited the enzyme activity with the percentage of 17, 26, 32, 37, 51 and 71 respectively. Also, when different concentrations of amaranth and barley extracts were assayed against α-amylase, a concentration dependent trend was observed. Also, amaranth and barley seed extracts had an optimum pH and temperature inhibition of 10 and 35 °C which are the optimum pH and temperature for the activity of this enzyme in the in vitro condition. Thus, it is concluded that amaranth seed proteins are potentially good for detailed investigation in order to get a clear picture of its active compound(s) and its structure-function relationship.