author(s)
Ayesha Zaman, Karishma Noor, Javid Khan, Muhammad Hashim Raza, Sarfraz Hussain, Muhammad Ayub Jadoon, Anees Fatima, Asima Bakht, Raheem Bahadar, Muhammad Usman, Hameed Ur Rehman and Muhammad Abdul Rehman

abstract
The toxoids are prepared from C. tetani toxins b inactivating them using formalin. The toxins were extracted from C. tetani by culturing them in broth and then incubated in fermentation medium to produce the toxins. The toxins were then filtered by use of filter paper. In this study the effect of toxins was observed on mice’s by injecting them intramuscularly. The toxin shows no affect when injected in low dose such as 0.1 micro liters but when the doses increased the symptoms starts to appear. The symptoms of toxin on mice’s were muscle spasm, paralysis and eventually death for leaving them untreatable. The toxoids prepared from these toxins were also tested on rabbits for checking its potency. The vaccinated animal sera was then taken and checked the immune response i.e. antitoxins in the sera of vaccinated animals. As doses of toxoids increased the immune response appeared was high. Thus these toxids can b use as a vaccine for the treatment of tetanus.