Activity of midgut amylase in Aeolesthes holosericea Fabricius (Coleoptera: Cerambycidae)
Patil NK, Raut GA and Gaikwad SM
Partial characterization of amylase from the larval and adult midgut of Aeolesthes holosericea was studied. High enzymatic activity was found at pH 5.2 and 35 °C in larva where as in adult it was at pH 5.4 and 40 °C. The linear period for enzymatic activity was 15 min in both larva and adult. The 50% inhibition at 55 °C was 1.5min and 4 min in larva and adult respectively. The Km values for larva and adult were 0.777% and 0.115% respectively. Findings of the present study would be utilized in to understand digestion process in insects and to formulate control strategies against wood boring insects.