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Journal of Entomology and Zoology Studies
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ISSN Print: 2349-6800 | ISSN Online: 2320-7078

Journal of Entomology and Zoology Studies

2016, Vol. 4, Issue 5
Evaluation of efficiency of four DNA extraction methods in Apis mellifera L. (Hymenoptera: Apidae)

Mehdi Modabber, Javad Nazemi Rafie and Jafar Abdollahzadeh

Quality and quantity of DNA extraction is important in molecular studies. In this research, four methods were evaluated in order to choose the most appropriate way in DNA extraction. DNA extraction methods included the optimal salt, phenol – choloroform, CTAB and CTAB + SDS. The quantity and quality of DNA extracted were compared with spectrophotometry and agarose gel respectively. Results indicated that CTAB+SDS method with the average purity of 1.89 (absorption ratio 260nm/280nm) was better than to other methods for DNA extraction in Apis mellifera.
Pages : 576-578 | 954 Views | 40 Downloads
How to cite this article:
Mehdi Modabber, Javad Nazemi Rafie, Jafar Abdollahzadeh. Evaluation of efficiency of four DNA extraction methods in Apis mellifera L. (Hymenoptera: Apidae). J Entomol Zool Stud 2016;4(5):576-578.
Journal of Entomology and Zoology Studies
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