author(s)
SB Bansode and RD Patil

abstract
Azadirachtin derived from neem (Azadirachta indica A. Juss) is a very effective and extensively used target specific and comparatively less toxic pesticide. Present work is carried out to determine 96 hours LC₅₀ value and safe concentration of Bioneem to fresh water fish Gara mullya (Sykes). The biostatic assay of neem based pesticide, Bioneem has been carried out to determine 96 hours LC₅₀ values at different exposure period for fresh water fish G. mullya. Estimated LC50 values at 24, 48, 72 and 96 hours of exposure to Bioneem are 334.58 ppm, 288.66 ppm, 222.53 ppm and 167.45 ppm respectively. G. mullya exposed to Bioneem (Azadirachtin) shows toxic effect and neem based pesticides may cause significant death of fishes when exposed for longer duration.