A study of the malaria transmission was carried out for the first time in the Manoka Island and Youpwè mainland area in Douala. Mosquitoes were sampled by human landing catches simultaneously indoor and outdoor in December 2013 and April 2014. Collected mosquitoes were morphologically identified and anopheles ovaries were dissected for parity determination. Legs and head-thorax of each dissected anopheles specimen belonging to Anopheles gambiae complex were used for molecular identification and infectivity status determination. A total of 2,827 female mosquitoes belonging to Anopheles, Culex and Aedes genera were collected in both localities. Anopheles gambiae complex and Culex pipiens was dominant respectively in Manoka (81.1%) and Youpwè (77.9%). In both localities, Anopheles gambiae s.l. had a high tendency to exophagy. Only Anopheles coluzzii was found infected by Plasmodium falciparum. Between Manoka and Youpwè, there was no significant difference in the infection rate and the entomological inoculation rate.