Toxicity assessment of the methanol extract from Elaeagnus angustifolia against larvae of Drosophila melanogaster meign (Diptera/Drosophilidae)
Syed Ishtiaq Anjum, Saber Husain, Hikmat Ullah Khan, Mohammad Attaullah, Sajid Rajput, Ayesha Haleem Shah, Islam Dad Buneri and Abdul Haleem Shah
The aim of the present study was to evaluate the toxicological potential of the methanol extract of Elaeagnus angustifolia plant against the 3rd instar larvae of Drosophila melanogaster megin (Diptera/Drosophilidae) by contact method. The larvae were exposed to the treatment of the studied extract for 24, 48, 72, 96 and 120 hours intervals. The mortality rate of larvae, retrieval of pupae and emergence of adults were noted at each interval of treatment. The obtained results indicated that methanol extract of E. angustifolia possess toxic potential against D. melanogaster larvae. With 1, 2 and 3% concentration of the studied extract, mortality rate was found to be 10, 10, 10 (for 24 hours), 10, 30, 90 (for 48 hours), 10, 50, 90 (for 72 hours), 10, 60, 90 (for 96 hours) and 10, 60, 90 (for 120 hours) respectively. The mortality rate of the larvae indicated that toxicity of the extract increases linearly with the increase of concentration and time period of treatment. This extract can be used against dipterous larvae and for the control of insect pests. The use of botanical extracts as a tool for biological control of pests is environment friendly and has no impaired health risks unlike those associated with chemical control measures. In this way, it is concluded that extract of E. angustifolia has toxic potential and can be effectively used for the control of insect pests.
Syed Ishtiaq Anjum, Saber Husain, Hikmat Ullah Khan, Mohammad Attaullah, Sajid Rajput, Ayesha Haleem Shah, Islam Dad Buneri, Abdul Haleem Shah. Toxicity assessment of the methanol extract from Elaeagnus angustifolia against larvae of Drosophila melanogaster meign (Diptera/Drosophilidae). J Entomol Zool Stud 2017;5(1):217-220.