author(s)
Mokosuli Yermia Semuel, Rudi Alexander Repi and Rantje Lilly Worang

abstract
This current research aims to find the characteristics and composition of the crude venom Apis dorsata Binghami, its activity of free radical DPPH scavenging, and cytotoxic activity. The analysis of honey bee venom peptide composition was performed using sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE). Phytochemical contents were analyzed using Harborne method, antioxidant activity was measured using DPPH free radical scavenging method and the anticancer activity in vitro was analyzed for cytotoxic effects on cancerous cells of murine leukemia p388 cell line. The results showed that bee venom AD1 has 5 bands of molecules with a molecular weight of 33,53 kDa; 32,21 kDa; 21,51 kDa; 6.1 kDa and 2.67 kDa; AD2 has molecules of 33,52 kDa; 21,51 kDa; 14,43 kDa; 6,14 kDa and 2.43 kDa. Apis dorsata crude venom of Minahasa in North Sulawesi contains hyaluronidase/phospholipase A; phospholipase; lysophospholipase or protease inhibitor 5 antigens, and melitin. An unknown tupe of peptide was also identified on fourth band. The analysis of phytochemicals revealed that the crude venom also contains flavonoids and polyphenols. AD1 antioxidant activity was present (IC₅₀:103,28 ppm), as well as the AD2 (IC₅₀:139,13 ppm) compared to control BHT (IC₅₀:142,38 ppm). Cytotoxic activity was observed in murine leukemia P388 cells by AD1 (IC₅₀:36,12 µg/ml), AD2 (IC₅₀:48,59 µg/ml) and positive control Canamycin (IC₅₀^:42,06 µ g/ml). Thus, Apis dorsata Binghami crude venom from Minahasa, North Sulawesi is potential to be developed as a source of bioactive antioxidant and anticancer.