Molecular identification of Peste-des-petits-ruminant virus from goats of Assam state of North-East India
SS Begum, G Mahato, D Muthuchelvan, D Chaudhary, M Hussain, N Nashiruddullah and KK Rajak
Peste des petits ruminants (PPR), which literally means “Plague of small ruminants” is an economically important disease of sheep and goats that is highly contagious with morbidity and mortality rates being as high as 100 per cent and 90 per cent, respectively. The present study was undertaken as a part of PhD research work to identify Peste-des-petits-ruminant virus from suspected PPR outbreak sample in goats of Assam state of North-East India.Five clinical samples were collected from a PPR suspected outbreak from goats of Darrang district of Assam, and the samples were tested by PPR Sandwich ELISA kit developed by Division of Virology, IVRI, Mukteshwar, Uttarakhand, India. Out of the five samples, one sample was positive for PPRV, and the viral RNA was isolated from the sample using Trizol method. Later the RNA from the sample was subjected to direct cDNA by RT-PCR, and this cDNA was used for expression of N (368 bp) and F (372 bp) gene of PPR virus using custom designed primers. Expression of the N and F gene confirmed the presence of PPRV in the clinical materials and war rented for quick action to be taken for control of the disease.
SS Begum, G Mahato, D Muthuchelvan, D Chaudhary, M Hussain, N Nashiruddullah, KK Rajak. Molecular identification of Peste-des-petits-ruminant virus from goats of Assam state of North-East India. J Entomol Zool Stud 2017;5(2):1366-1368.