In this study, it is purposed that the protective effect of the melatonine (MEL) is examined against the toxic effect of the CCl4 to the gastrocnemius muscle. The rats were separated into 4 groups that were control groups (Group 1, Group 2), CCl4 (Group 3) and CCl4 + MEL (Group 4). The tissues were processed the routine preparation processing for the light microscope. The tissues were stained respectively with hematoxylin-eosin, Masson-trichrome and Periodic Acid- Schiff. When the tissues were stained with histological stains whole of the control groups was regular. When the tissues were stained with hematoxylin-eosin; and in the group (group 3), in the muscle fibers there were fusion in places, hypertrophy and orientation disorder. In the group (Group 4) was observed that the muscles were close to the control group. When the tissues were stained with the Masson-trichrome, in the group 3, it was observed that the collagen fibers were increased in the connective tissue. In the CCl4 group which was applied the melatonine, it was observed that in the collagen fibers there were decreased. In addition to these, in PAS staining all groups were seen PAS positive.