author(s)
Hidayat khan, Ikram ullah, Qazi Sami Ul Haq, Ziafat Rehman, Shafiq ur Rehman, Muhammad Ayub Jaddon, Ahsan Ullah, Azam Hayat, Sidra Khan, Mujaddad Ur Rehman, Inam Ullah and Dr. nighat sultana

abstract
The purpose of the present study was to confirm the presence of PLRV by DAS-ELISA and RT-PCR. For this the present study undergo serological and molecular detection of potato leaf roll virus (PLRV) by studying PLRV on the basis of symptomlogy, by serological detection (DAS-ELISA) and amplification of coat protein gene by RT-PCR. Ten (10) samples of potato were tested and 8 were found to be infected either by a single virus or more than one virus. Eighty (80)% infection was observed by DAS-ELISA and the resulted cDNA was used in PCR reaction using gene specific primer pair an amplified product of approximately 626 bp was observed.