Identification and characterization of soil bacterial isolates and their role in the degradation of flubendiamide insecticide in the artificial medium
Dinesh Rajaram Hegde, T Manoharan and R Sridar
Soil bacterial colonies capable of degrading flubendiamide (FBD) were isolated from three different ecosystems such as cotton, cabbage and paddy fields, Coimbatore, Tamil Nadu followed by enrichment technique. Totally, six colonies were isolated and were morphologically and biochemically tested. Among six bacterial isolates, three isolates named as FBD-1, FBD-2 and FBD-6 were found to be recorded maximum growth in the medium supplemented with flubendiamide as sole carbon source and hence taken for further dissipation studies. Highest dissipation of flubendiamide was recorded in the mineral salt medium inoculated with FBD-2 (92.14%) followed by FBD- 1 (88.13%) compared to 65.57 percent in the control after 15 days of inoculation. The bacterial isolates viz., FBD-1, FBD-2 and FBD-6 were later identified as Micrococcus sp., Corynebacterium sp. and Pseudoxanthomonas sp., respectively through molecular characterization by 16S rRNA gene sequencing. These results highlight the potential of bacterial cultures to be used in the cleanup of pesticide contaminated sites in the environment.
Dinesh Rajaram Hegde, T Manoharan, R Sridar. Identification and characterization of soil bacterial isolates and their role in the degradation of flubendiamide insecticide in the artificial medium. J Entomol Zool Stud 2017;5(3):1383-1387.