author(s)
Ali Bouaziz, Khedidja Amira, Nour El-Houda Djeghader, Linda Aïssaoui and Hamid Boudjelida

abstract
The present study was conducted under laboratory conditions and consisted to evaluate the lethal concentrations (LC₅₀ & LC₉₀) effects of an agonist of the molting hormone, the halofenozide (RH-0345), on several physiological and biological aspects (developmental duration, morphology, metabolites and potential reproduction) after treatment of newly ecdysed fourth instar larvae of mosquito species, Culex pipiens. The results showed that the RH-0345 induced a decrease of the developmental duration of the treated larval stage with morphological aberrations. The duration of control ones was 7.5 days whereas for treated series, it was 6.66 and 6 days for LC₅₀ and LC₉₀, respectively. The biochemical assays showed that the product has reduced significantly the amount of the metabolite contents; carbohydrates, lipids and proteins. The potential of reproduction of adult females emerged from treated fourth instar larvae, was affected and the number of laid eggs, hatching and fecundity were decreased compared to the control.