The present study the effect of okra seed supplementation on serum lipid profile of hypercholesterolemiainduced rat in Varanasi city at Banaras Hindu University Department of Zoology, Uttar Pradesh for a period of 42 days during year 2017. Okra seed (dry) were, grinded and administered at dose of 250grams and 500 grams as low dose and high dose. Administration of okra seed powder of 250 gm (low dose) and 500 grams (high dose) for 42 days produces significant (P<0.001) reduction of serum LDL cholesterol and in body weight reduction in hyperlipidemia. The present study conform that okra seed powder is effective for lipid lowering.