author(s)
Emmanuel O Ogueji, Christopher D Nwani, Stanley C Iheanacho, Christian E Mbah, Ogochukwu C Okeke and Ibrahim B Usman

abstract

The present study evaluated the acute toxicity of Ibuprofen (IBU) on lipid peroxidation, antioxidant enzyme system and biochemical parameters of Clarias gariepinus juveniles. The 96 h LC₅₀ of IBU was 0.38 mg·L^-1, in a semi static system with significant difference (p< 0.05) in LC_10-90 values obtained for different durations of exposure. Catalytic activities of glutathione reductase (GR), Superoxide dismutase (SOD), glutathione peroxidase (GPx), catalase (CAT) and lipid peroxidation (LPO) were evaluated. Biochemical parameters such as serum glucose, protein, alkaline phosphatase (ALP), aspartate aminotransferase (AST) and alanine aminotransferase (ALT) were also measured. LPO (130.62%), SOD (76.95%), CAT (69.09%), GR (47.04%) and the liver function parameters ALP (37.70%), AST (35.55%) and ALT (69.45%) were all significantly (p<0.05) elevated when compared with control indicating oxidative stress and hepatic damage. Glucose increased in the lowest concentration of 0.28mg/L (9.20%) and decreased in the higher concentrations 0.33mg/L-0.48mg/L (21.10%). Protein decreased (46.38%) and GPx decreased dose dependently after 24h exposure. However, decreases in day 2-4 appeared with mixed trend. Formation of oxygen radicals may have contributed to the toxicity of IBU.