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Journal of Entomology and Zoology Studies
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ISSN Print: 2349-6800 | ISSN Online: 2320-7078

Journal of Entomology and Zoology Studies

2017, Vol. 5, Issue 4
Sequence analysis of mitochondrial COII gene fragment from five Culicinae mosquito species

Devinder Singh, Navpreet Kaur Gill and Navneet Kaur Rai

Sequence analysis of the mitochondrial COII gene has been used for molecular taxonomy in many insects and to reveal phylogenetic relationship among them. A ~500 to 550bp sequence of mitochondrial Cytochrome Oxidase II (COII) gene has been analyzed to construct molecular database and to establish the phylogenetic relation among the five Culicinae mosquito species (Aedes vittatus, Aedes aegypti, Culex bitaeniorhynchus, Culex vishnui and Culex quinquefasciatus) from the state of Punjab and adjoining areas (India). The sequences were found to be A+T rich and in substitution the rate of transitions was higher than the rate of transversions. Individuals of the same species grouped closely together in a Neighbor-Joining tree regardless of collection site. Conspecifics showed <2% divergence (range = 0% to 1.7%), whereas interspecific divergence was >2% with K2P (range = 4.1% to 15.0%).
Pages : 1386-1390 | 532 Views | 17 Downloads
How to cite this article:
Devinder Singh, Navpreet Kaur Gill, Navneet Kaur Rai. Sequence analysis of mitochondrial COII gene fragment from five Culicinae mosquito species. J Entomol Zool Stud 2017;5(4):1386-1390.
Journal of Entomology and Zoology Studies
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