Detection of Cysticercus cellulosae in slaughtered pigs through meat inspection and confirmation by PCR assay
Koushik Kakoty, Poznur Hussain, Saidul Islam, Razibuddin Ahmed Hazarika, Gouranga Mahato and Manoj Kumar Kalita
A total of 316 pig carcasses were examined through meat inspection in different market places of Sivasagar district of Assam, India for a period of one year from April 2015 to May 2016. Out of which, 4 (1.27%) were found positive for porcine cysticercosis with visible cysts. The highest density of Cysticercus cellulosae was found (11.40±0.67) × (4.8±0.58) mm in skeletal muscles. Polymerase chain reaction (PCR) assay was performed to confirm Cysticercus cellulosae and to validate the results of meat inspection. Oligonucleotide primers targeting against the large subunit rRNA gene (TBR primers) of Taenia solium were used in this study. On reactivity in PCR test, the TBR primers yielded products of 286bp in cysticercosis positive cases.