author(s)
Dr. Parnashree Mukherjee, Anu Mishra and Anshul Sinha

abstract
The basic aim of the current study was to investigate the physiological stress indicators in haemolymph of insects of domestic gardens and road sides and relative analysis of the immunocompetence status of insects of roadside and domestic gardens of Jabalpur city to keep a check on their biodiversity loss and to assess their role in maintaining ecological balance. During the present study period insect samples were collected from 2 busy roadsides habitat and 10 domestic gardens of Jabalpur city to investigate the immunocompetence status of the insects of road sides and domestic gardens of Jabalpur city from July 2015 to September 2016. Haemolymph of insects were collected in ice cooled 1.5 ml microcentrifuge tubes prefilled with 300µl sodium cacodylate based anticoagulant followed by relative analysis of various commonly used physiological stress indicators in insect haemolymph such as total and differential haemocyte counts, total protein composition (in terms of refractive index),clotting capacity of hemolymph and protein profile to portray the immunocompetence status of the insects of road side in comparison to those found in the domestic gardens. During the current estimation of Heamolymph proteins of insects of all the 05 orders of road side insects by SDS-PAGE followed by Commassic blue and silver staining revealed the presence of the heat shock proteins in the insects which help to increase their resistance against adverse conditions of the roadsides. The present study indicated that the high values of THC of the adult insects of both roadsides and domestic garden correlated with less coagulation time which serve as indicator of good immunocompetence status. The present comparative study of insects of both the habitats indicates that the haematological parameters like DHC%, THC, serum protein content and clotting time of insects of domestic gardens were found to be lesser than that of the road side insects which indicates poor immunocompetence status of insects of domestic garden in comparison to the insects of roadsides of Jabalpur city.