Compatibility of Metarhizium anisopliae with neem derivatives in vitro condition
Kelwatkar NM, Kankale MD and Das SB
The present investigation was carried out during rabi 2013-2014 at the Entomology laboratory, College of Agriculture JNKVV, Jabalpur at Completely Randomized Block Design (CRBD). The experiment on compatibility studies was undertaken on four treatments and five replication with neem derivatives. The effect of neem derivatives on the mycelial growth of M. anisopliae was conducted in In vitro. Among the neem derivatives tested Neem soap 0.5% (w/v) (half recommended dose) was found to be most compatible with least inhibition growth followed by Neem oil 2.5% (v/v) and NSKE 2.5% (w/v). Although the different neem derivatives tested in the present investigations slightly inhibited the growth of M. anisopliae in poisoned media, the combined use of the fungus and neem derivatives cannot be completely ruled out. All the neem derivatives tested have been combined with entomopathogenic fungi for obtaining better control of pod borer in chickpea considering this it is worth exploring the effect of these neem derivatives at sub lethal dose with fungus for an enhance result over pest control.