Contact: +91-9711224068
Journal of Entomology and Zoology Studies
  • Printed Journal
  • Indexed Journal
  • Refereed Journal
  • Peer Reviewed Journal

P-ISSN: 2349-6800, E-ISSN: 2320-7078

Journal of Entomology and Zoology Studies

2017, Vol. 5, Issue 4
Variation in brush border membrane vesicle receptors and activity of proteolytic enzymes in relation to toxicity of Bt toxins to legume pod borer, Helicoverpa armigera (Lepidoptera: Noctuidae) larvae

Inakarla Paramasiva, Pulipaka Venkata Krishnayya and Hari Chand Sharma

Mechanism involved in development of resistance in Helicoverpa armigera larval populations from nine locations was evaluated at International Crops Research Institute for the Semi Arid Tropics (ICRISAT), Patancheru, Telangana, India during 2011 and 2012 by performing; a) in-vitro binding assays with Brush border membrane vesicle receptors and trypsin activated Bt toxin Cry 1Ac and b) by performing SDS-PAGE with activated toxin and gut juices of H. armigra populations form nine locations.
PAGE with activated toxin and gut juices of H. armigra populations form nine locations.
The present study results demonstrated that resistance/lower sensitivity of Mahabubnagar and Parbhani H. armigera larval populations (LC90 values 10.2851 and 15.6230 µg ml-1) to Bt toxins was due to reduction in proteolytic activity of gut extract results in production of less activated toxin and degradation of the activated toxin to a relatively less toxic product. Resistance of Nanded population (LC90 value 13.7219 µg ml-1) was due to low amount of the activated toxin produced from the protoxin. In case of Bidar and Kurnool populations, their relative sensitivity to Bt (LC90 values 4.0434 and 5.7445 µg ml-1, respectively) was due to high binding ability of activated toxin to Brush border membrane vesicle receptor proteins of midgut epithelial cells (137 7 135 µg g-1).
Pages : 1734-1739 | 1004 Views | 96 Downloads


Journal of Entomology and Zoology Studies Journal of Entomology and Zoology Studies
How to cite this article:
Inakarla Paramasiva, Pulipaka Venkata Krishnayya, Hari Chand Sharma. Variation in brush border membrane vesicle receptors and activity of proteolytic enzymes in relation to toxicity of Bt toxins to legume pod borer, Helicoverpa armigera (Lepidoptera: Noctuidae) larvae. J Entomol Zool Stud 2017;5(4):1734-1739.

Call for book chapter
Journal of Entomology and Zoology Studies
Please use another browser.