Karyo-morphometric studies of Chironomus circumdatus from Manipur, India
Dhananjoy Singh Chingangbam, Subhadra Devi Wahengbam and Ishwar Haobam
The present investigation was conducted to study the karyo-morphometric characteristics of Chironomus circumdatus from Manipur during July 2016. The specimens of the Chironomus were collected from sewage drainage located along the road. The larvae were reared in the water from the site till the adults were collected and reared again for the eggs. The study was carried out using the eggs, larvae, pupal exuviae and adults. The confirmation was mainly done from the male adult fly after treating with KOH. The results showed that superior volsella was D type curved at tip in the species of Chironomus circumdatus. The polytene chromosomes were four (i.e. 2n=8) with the pseudothummi-cytocomplex combination AE, BF, CD, G. Nucleoli in arm B and C, with a small one also present sub-terminal in arm G. Not much variation could be observed in the specimens from Manipur both in morphology and polytene chromosomes from the rest of the world. Further studies of the Chironomus from Manipur will be much be an interesting regarding the organisms, distribution, evolutionary and adaptation.