author(s)
M Ramar, P Manonmani, P Arumugam, S Kamala Kannan, R Raskin Erusan, N Baskaran and K Murugan

abstract
The present study was contacted to prepared novel nanoemulsion formulations (NEFs) from the essential oil (Ocimum sanctum) as a nano-insecticidal formulation with different ratios of emulsifier, Stabilizer and water. The activities of knock down and adulticidal effect of the formulated nanoemulsion formulations was evaluated against Aedes aegypti and Culex quinquefasciatus. The oil in water (o/w) emulsions was prepared using with oil, emulsifier, Stabilizer and water. The larger droplet size decreased into a smaller size with increase in the concentration of emulsifier. The viscosity and thickness of the nanoemulsion decreased with increasing of concentration of emulsifier. Knock down (%) and KD₅₀ and KD₉₀ of the nanoemulsions against Ae. aegypti and Cx. quinquefasciatus more effect was recorded for F2 36.4±1.73, 48.2±2.64, 67.5±3.23, 85.4±1.45 and 98.2±3.43; and 42.3±2.73, 59.3±1.65, 78.4±0.14, 92.6±3.85 and 100±0.00 respectively. Also, KD₅₀ and KD ₉₀ values were noticed for F2 7.01 and 29.94; 4.05 and 20.88, respectively. The adult mortality was recorded on A. aegypti and C. quinquefaciatus in F2 17.5±0.75, 22.5±1.25, 33.7±0.87, 52.5±1.50 and 71.2±1.25; 23.7±1.125, 30.2±1.25, 43.7±0.37, 61.2±2.185 and 82.5±1.75, respectively. The LD₅₀ and LD₉₀ values were observed in F2 28.60 and 69.82; 20.09 and 57.13, respectively. According to Knock down (1 h) and mortality (24 h) level, this nanoemulsion was showed potential insecticidal agent against Aedes aegypti and C. quinquefaciatus adult. The present study permitted the development of low-cost eco-friendly green Insecticidal nanoformulations with potential adulticidal activity, using a nano-technology based approach.