author(s)
Asifa Wali, Syed Mudasir Ahmad, Rafia maqbool, Masood-ul Hassan Balkhi

abstract
DNA material was collected from the three cyprinid species viz. cyprinus carpio var. communis, cyprinus carpio var. specularis and Carassius carassius, found in different water bodies of Kashmir valley. To analyze the population level genetic variations molecular approach viz restriction digestion of mitochondrial Cyt b (PCR-RFLP) was used for 25 samples of each species. Further Experiments were conducted to assess the efficiency of PCR-RFLP marker systems as molecular tools for genetic variation of three cyprinid species. Total genomic DNA was extracted and used for PCR amplification of the Cyt b region. The purified PCR products were subjected to single digestion with MboI, MspI, BsuRI and Hin6I enzymes and visualized on 1.5% agarose gel. The phylogenetic analysis demonstrated that scale carp (Cyprinus carpio communis) is the closest to mirror carp (Cyprinus carpio specularis) than from crucian carp (Carassius carassius).