Immune response of rabbits immunized by killed whole cell sonicated E. coli 0157 antigen and crude Klebocin
Zahraa Eisaa Sadeq and Ikram AA Al-Samarrae
This present research was aimed to estimate the immune responses of rabbits immunized by KWCSA-E.coli 0157 and Klebocin during the period from November-2016 to May-2017. Thirty rabbits of both genders were randomly divided into six equal groups (5 animals for each group). Three types of tests were performed which were Skin Test (The delayed type hypersensitivity (DTH)), Tube Agglutination Test and ELISA. The Skin Test was performed at 18 days post immunization. The results showed an elevation in the means of erythema at 24hr of the 1st, 2nd, 3rd,4th and 5th groups which were 9.70, 8.90, 8.20, 6.00± 0.15 and 5.60 mm respectively. There was also a significant increasing (P<0.05) in the diameter of induration of the same sequence groups (8.30, 8.00, 7.60, 5.00 and 4.50 mm). The results of tube agglutination test showed at 20 days the highest titration was (1120.00) in the first group, while the lowest titer was 160.00 in the fifth group with significant differences (P˂0.05). On the other hand ELISA showed elevation at 20 days in both of IL-2 (180.82) and IL-6 (107.69) levels in first group whereas the lowest concentration at 60 days post immunization in all groups, with significant differences (P˂0.0 5). The results of the present study indicated that there was an interaction between both antigens which could enhance the humoral and cellular immune response.