Detection of Mycotic caprine mastitis through studying of Oxidant/Antioxidant state
Shaimaa N Yassein, Luma W Khalil and Jenan Mahmood Khalaf
The present study was conducted to evaluate the antioxidant profile in cases of subclinical mastitis (SCM) in goats during the period from June 2015 to March 2016. This study included 9 lactated goats which were divided into 3 groups. The first one was immunized by HKCn Ag in the right mammary gland. The 2nd group was infected by virulent Cryptococcus neoformans strain in the right mammary gland, while the 3rd group left as control. The blood and milk samples were collected at 0, 3d, 1st, 2nd,3rd and 4th week for determination of MDA and GSH level. The objective of this study is to determine the the possibility of using oxidative product levels (MDA and GSH) as markers for the diagnosis of SCM in goats. The results showed that the differences in MDA level were not significant in milk. While in the serum, the MDA level showed a significant decreasing (P<0.05) in the 1st group compared with the 2nd group, and both of these groups were higher than the control group. The GSH level increased significantly (P<0.05) in the immunized group as compared with the mastitic group, and both of these groups showed significant (P<0.05) differences as compared with healthy group. In conclusion: this study indicated that the measurement of MDA and GSH activities in milk and serum appears to be a suitable diagnostic method for identifying SCM in dairy goat.