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Journal of Entomology and Zoology Studies
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ISSN Print: 2349-6800 | ISSN Online: 2320-7078

Journal of Entomology and Zoology Studies

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2017, Vol. 5, Issue 5
Studies of isolation, identification and differentiation of vibrio species from Catla catla fish by cultural and multiplex PCR methods

Rajesh Kumar Sahu and Krishnaiah Nelapati

The present study was undertaken to standardize Multiplex PCR assay for detection and differentiation of Vibrio cholera, Vibrio parahaemolyticus and Vibrio vulnificus from freshwater fish catla catla and to compare with conventional cultural methods. The samples were collected from local markets of Hyderabad from January to May 2017. The specificity for Vibrios was tested using primers from gyrB gene which gave amplification product of 493 bp only for Vibrio organisms whereas non-Vibrio organisms did not yield any products. Primers for pntA gene was used for differentiation of Vibrio species which gave a specific amplification products of 338, 409 and 656 bp products for Vibrio cholera, Vibrio parahaemolyticus and Vibrio vulnificus respectively. Out of 150 markets raw samples 80 samples were positive for Vibrio spp by m-PCR, out of which 47, 21 and 12 were positive for V. cholerae, V. parahaemolyticus and V. vulnificus respectively, whereas cultural methods gave 72 positive for vibrio species. The mean counts of V. cholerae, V. parahaemolyticus and V. vulnificus were 7.1 x105 cfu, 1.07x103 cfu and 5.8x102 cfu in catla fish.
Pages : 1098-1101 | 467 Views | 17 Downloads
How to cite this article:
Rajesh Kumar Sahu, Krishnaiah Nelapati. Studies of isolation, identification and differentiation of vibrio species from Catla catla fish by cultural and multiplex PCR methods. J Entomol Zool Stud 2017;5(5):1098-1101.
Journal of Entomology and Zoology Studies
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