Detection of aflatoxin B1 from fodder crops in ruler area around Baghdad city in Iraq and studied the toxicity effect of A. flavus in mice
Mohammad Hussain Abad and Dr. Zainab Abdul-Zahra Al-haddad
The present study was aimed to detection of aflatoxin B1 from Fodder crops which included maize and sesame in rural area around Baghdad city and studied the toxicity effect of A. flavus in mice fed on diet contaminated with spores of toxigenic A. flavusafter 30 days of experiment, fifty (50) sample from maize and sesame were collected from the ruler areas around Baghdad city which were (Al Tarmia, Abu Ghraib, Mahmoudiya, Alabegi and Taji), so ten (10) sample from maize and ten (10) sample from sesame were obtained from each of the areas above in one (1) kilogram from each sample during four months begging from (November/2016 till march 2017)., the detection percentages of aflatoxin B1 from maize and sesame were 50% and 24% respectively, this study was conducted with experimental infection included diet of mice on feed contaminated withA. flavus in three different doses, the histopathological examination had been done on spleen, lung, small intestine and liver of mice in experiment, so the spleen appeared with different types of inflammatory cells and lymphocytes with highly infiltration with macrophages which appeared in diffuse and nodular forms, the lung characterized by infiltration with inflammatory cells and macrophages as well as breaking of epithelial lining the bronchiole, an addition to intestine revealed highly infiltration with inflammatory cells with irregular in intestinal villi while liver showed sever degenerative in the hepatocytes with payknosis in their nuclei in addition to presence of lymphocytes and kupffer cell.
Mohammad Hussain Abad, Dr. Zainab Abdul-Zahra Al-haddad. Detection of aflatoxin B1 from fodder crops in ruler area around Baghdad city in Iraq and studied the toxicity effect of A. flavus in mice. J Entomol Zool Stud 2017;5(6):379-383.